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. Author manuscript; available in PMC: 2010 Mar 22.
Published in final edited form as: Toxicol Pathol. 2009;37(7):969–982. doi: 10.1177/0192623309350475

Figure 3.

Figure 3

Representative renal sections stained with the fluorescent dye DHE (A) and quantification of nuclear ethidium staining (B) illustrate increased renal reactive oxygen species generation in medroxyprogesterone acetate–treated AST rats. Reactive oxygen species-dependent DHE oxidation yields a red fluorescence signal that localizes to nuclei. Nonoxidized DHE appears as a blue cytosolic fluorescence signal. (n= 4 animals/group; *p < 0.05 vs. all groups). Abbreviations: ovx (ovarectomy); AST (aldosterone-salt treatment); DRO (drospirenone); MPA (medroxyprogesterone acetate); E2 (17β-estradiol); SPIRO (spironolactone).