Table I.
Phenotype of monocytes, macrophages, and dendritic cellsa
Relative Expression Level of Phenotypic Markers |
||||
---|---|---|---|---|
Monocytes | Macrophages | Immature DC | Mature DC | |
MHC II | ++ | ++/+++ | +++ | +++ |
CD3 | − | − | − | − |
CD11c | + | + | + | + |
CD14 | ++ | + | +/− | − |
CD40 | + | + | + | ++ |
CD54 | + | + | + | ++ |
CD80 | − | + | + | +/++ |
CD83 | − | − | − | + |
CD86 | + | + | + | ++ |
DC-SIGN | − | − | + | + |
Human monocytes, macrophages, and DCs were stained with fluorophore-conjugated Abs to the listed cell surface Ags and assessed by flow cytometry. The relative expression level for each marker is given and, in all cases, was consistent with the literature.