Table 2.
Features of the 18 TvBspA proteins encoded by scaffold DS113361
| Locus taga | Alignb | Protein length |
LRRc start |
LRRc end |
Pa/Prc | E-valued | TMDe position |
Notable featuresf |
|---|---|---|---|---|---|---|---|---|
|
Subfamily #22 (14 entries) |
||||||||
| TVAG_133050 | 110 | 837 | 15 | 796 | 1 | 2.0E-69 | Conserved C-terminus |
|
| Subfamily #200 | ||||||||
| TVAG_133260 | 649 | 285 | 56 | 273 | 0 | 3.0E-21 | ||
| TVAG_133330 | 650 | 666 | 23 | 656 | 0 | 7.0E-43 | ||
| TVAG_133300 | 651 | 566 | 65 | 517 | 1 | 4.0E-46 | 468-490, 539-561 |
|
| TVAG_133380 | 652 | 174 | 38 | 173 | 0 | 9.0E-16 | ||
| TVAG_133420 | 653 | 392 | 66 | 390 | 0 | 3.0E-29 | SP | |
| TVAG_133230 | 678 | 205 | 3 | 201 | 1 | 9.0E-26 | ||
| TVAG_133270 | 679 | 205 | 3 | 201 | 1 | 9.0E-26 | ||
| Subfamily #228 | ||||||||
| TVAG_133390 | 680 | 638 | 3 | 555 | 2 | 4.0E-43 | ||
| TVAG_133240 | 681 | 601 | 5 | 358 | 0 | 3.0E-26 | 513-535 | Conserved CT |
| TVAG_133280 | 682 | 583 | 5 | 352 | 0 | 7.0E-26 | 495-517 | Conserved CT |
| TVAG_133310 | 683 | 595 | 4 | 382 | 1 | 4.0E-30 | 505-527 | Conserved CT |
| TVAG_133340 | 684 | 448 | 3 | 210 | 1 | 3.0E-17 | 358-380 | Conserved CT |
| TVAG_133430 | 685 | 734 | 3 | 474 | 1 | 2.0E-35 | 744-666 | Conserved CT |
| Subfamily #275 | ||||||||
| TVAG_133290 | 689 | 318 | 3 | 227 | 2 | 8.0E-34 | ||
| TVAG_133410 | 690 | 562 | 1 | 426 | 1 | 8.0E-40 | ||
| TVAG_133360 | 691 | 333 | 2 | 263 | 1 | 5.0E-26 | ||
| TVAG_133220 | 692 | 363 | 51 | 362 | 0 | 2.0E-31 | ||
aThe locus tag numbers give an indication of the proximity of the different TvBspA encoding genes on the scaffold, with an incremental of 10 for each neighbouring genes (see additional file 8, Figure S3). The numbers of entries for each of the four proteins subfamilies identified by CLUSS2 (additional file 1, Table S1) are indicated.
bAlignment position in the output file generated by ClustalW. The position of the 18 TvBspA proteins in the global TvBspA alignment (additional file 1, Table S1) is indicated.
cThe position of TpLRR inferred by BlastP (see Methods section). The numbers indicate the start and end of the TpLRR. The entries positive for either the TpLRR profile or pattern (Pa/Pr), or both, are indicated by a 1 and 2 respectively.
dE-values for the PSI-Blast search (2nd iteration) that followed a PHI-Blast (see Methods section).
eEntries with inferred transmembrane domains (TMD) are indicated with the shown start and end position of the TMD. All such entries had the N-termini with the TpLRR domain inferred to be "external" by two or more TMD predictors (see Methods section).
fTVAG_133420 has an inferred signal peptide (SP). The five entries from subfamily #228 with a TMD all share a similar cytoplasmic tail - see Figure 3.