Table 4.
Microarray data for 13 TvBspA with significant modulation in their mRNA concentration upon exposure to different iron concentration
| Locus tag | Annotationa | Subfamily membershipa, b | ESTa | Modul.c | p-value | qRT-PCRd |
|---|---|---|---|---|---|---|
| High iron culture condition | ||||||
| TVAG_397210 | BspA-like | #43, 10 memberse | 1 | 2.2 | 1.5 × 10-4 | 2.16 ± 0.002 |
| TVAG_441420 | BspA-like, SP, TMD | #374, singleton, divergent TpLRR | 1 | 1.55 | 8.4 × 10-3 | 2.02 ± 0.04 |
| TVAG_191490 | BspA-like, SP, PG, GRD | #168, 12 members | 1 | 1.29 | 5.5 × 10-3 | --- |
| TVAG_396970 | BspA-like | #44, 15 members* | 2 | 1.23 | 4.8 × 10-3 | --- |
| TVAG_080240 | BspA-like | #44, 15 members* | 6 | 1.18 | 8.5 × 10-3 | --- |
| Low iron culture condition | ||||||
| TVAG_093850 | BspA-like | #184, 3 members | 2 | -1.27 | 2.9 × 10-3 | --- |
| TVAG_299910 | BspA-like | #69, 3 members * | 16 | -1.25 | 6.8 × 10-3 | --- |
| TVAG_493590 | BspA-like | #384, 6 members | 4 | -1.24 | 3.7 × 10-3 | --- |
| TVAG_129450 | BspA-like, PG | #30, 15 members | 1 | -1.18 | 2.4 × 10-5 | --- |
| TVAG_530030 | BspA-like | #99, 2 members | 7 | -1.15 | 2.5 × 10-3 | -2.06 ± 0.02 |
| TVAG_341990 | BspA-like | #215, 3 members | 1 | -1.14 | 3.7 × 10-3 | --- |
| TVAG_176920 | BspA-like, TMD | #341, singleton, divergent TpLRR | 1 | -1.13 | 7.8 × 10-3 | --- |
| TVAG_235070 | BspA-like | #73, Singleton | 1 | -1.12 | 3.9 × 10-4 | --- |
| Control genes | ||||||
| TVAG_238830 | Hydrogenosomal malic enzyme subunit B | --- | --- | 1.67 | 1.5 × 10-4 | 2.51 ± 0.01 |
| TVAG_165030 | Cytosolic malate dehydrogenase | --- | --- | -1.33 | 2.8 × 10-4 | -1.62 ± 0.03 |
aSee additional file 1, Table S1 for full details. SP, signal peptide; TMD, transmembrane domain; PG, pseudogene; GRD, glycine-rich domain.
bSF, subfamilies identified with CLUSS2. *Related sequences identified by visual inspection of the alignment.
cPositive values indicate up-regulated genes and negative values down-regulated genes. All values are derived from quadruplets with indicated mean value (Log scale) and significance (p-values) of modulation.
dqRT-PCR was performed in quadruplets with indicated mean and standard deviations.