Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2010 Mar 23;4(3):e649. doi: 10.1371/journal.pntd.0000649

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.

This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration, which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.

PMC Copyright notice

The study was divided in three phases, each of them using different sample sets to address the suitability for the use of Lu. longipalpis salivary recombinant proteins as marker of human vector exposure. To establish the cut-off values form the first phase, 26 indiduals from a non-endemic area were tested for SGS and the recombinant proteins (see details in the methods section). In the first and second phases, samples were obtained from São Luis, Maranhão State, in northeastern Brazil, where VL is endemic and Lu. longipalpis is prevalent. To assess cross-reactivity, 40 samples from the VL endemic area who were positive for Lu. longipalpis anti-SGS were tested fom anti-SGS from Lu. intermedia, while other 40 samples from an endemic area of cutaneous leishmaniasis were used (Canoas, a rural village in Bahia, Brazil) were tested for anti-SGS from Lu. longipalpis and the recombinant proteins (dashed line box). The third phase used serum samples obtained from children residing in two other endemic areas for visceral leishmaniasis (Vila Nova and Bom Viver), in Raposa county, Maranhão State, Brazil. The study design details are described in methods. SGS: salivary gland sonitate; VL: visceral leishmaniasis.