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. 2010 Mar 23;4(3):e638. doi: 10.1371/journal.pntd.0000638

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This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.

This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration, which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.

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A, Two representative human sera from São Luis, a VL- endemic area where Lutzomyia longipalpis predominates, were screened against salivary proteins from Lu. longipalpis (LL), Lu. intermedia (LI), Lu. verrucarum (LV), and Phlebotomus perniciosus (PPe). B, Western blot analysis of salivary proteins from Lu. longipalpis using human sera from Canoa, a cutaneous leishmaniasis endemic area where Lu. intermedia predominates (CL endemic area; left bottom panel), or from São Luis where Lu. longipalpis predominates (VL endemic area). Sera from individuals from a non-endemic area were used as negative controls (CTL–). The profile of salivary proteins from Lu. intermedia recognized by a representative human serum (out of six tested ) from the Canoa endemic area (CL endemic area; right bottom panel).