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. 2010 Mar 23;4(3):e638. doi: 10.1371/journal.pntd.0000638

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This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.

This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration, which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.

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A, Flowchart describing the expression, purification and detection of recombinant sand fly salivary proteins from Lutzomyia longipalpis. B, Chromatogram of the purification of the LJM17 recombinant protein by HPLC-metal affinity chromatography. C, Detection of positive fractions for the LJM17 recombinant protein using sera from mice immunized with LJM17 plasmid. D, Silver-stained SDS-PAGE of LJM17 recombinant protein before (1) and after (2) HPLC purification.