Table 1.
primers and beacon probes used in this study:
| gene | Primer forward | Beacon probe | Primer reverse |
|---|---|---|---|
| MGMT METHYLATED | 5'-TTTCGACGTTCGTAGGTTTTCG+C-3' | 5'-FAM- CCGGAGCGTATCGTTTGCGATTTGGTGAGTGTGCTCCGG-BHQ1-3' | 5'-GCACTCTTCCGAAAACGAAAC+G-3' |
| MGMT UNMETHYLATED | 5'-TTTGTGTTTTGATGTTTGTAGGTTTTTG+T-3' | 5'-FAM-CCGGTGCTGTATTGTTTGTGATTTGGTGAGTGTGCACCGG-BHQ1-3' | 5'-AACTCCACACTCTTCCAAAAACAAAAC+A-3' |
| SNURF METHYLATED | 5'-GGATTTTTGTATTG+CGGTAAATAAGTA+C-3' | 5'-FAM- CCGGAGGGAGGTAGGTTGGCGCGTATGTTTAGCCTCCGG-BH1-3' | 5'-CGCTACAACAAC+GACAAACTTC+G-3' |
| SNURF UNMETHYLATED | 5'-GGATTTTTGTATTG+TGGTAAATAAGTA+T-3' | 5'-FAM- CCGGAGGGAGGTAGGTTGGTGTGTATGTTTAGGCCTCCGG-BH1-3' | 5'-CTCACTACAACAAC+AACAAACTTC+A-3' |
| SNURF FLANKING PRIMERS | GGGAGTT+GGGATTTTT+GTATTG | CTCCC+CAAACTATCT+CTTAAAAAAAA | |
(LNA nucleotides are preceeded by +; CpG discriminatory LNA nucleotides are in bold)