Table 2.
Primers' sequences
| mRNA | forward primer | reverse primer |
|---|---|---|
| HAX1 variant I | 5'-GACCTCGGAGCCACAGAGAT-3' | 5'-GGTGCTGAGGACTATGGAAC-3' |
| GenBank: NM_006118 | ||
| HAX1 variant II | 5'-GGACCTCGGAGCTTCAG-3' | 5'-TGACTCAGGACCTGGAAGTT-3' (semi-qPCR) |
| GenBank: NM_001018837 | 5'-CCATATCGCTGAAGATGCTA-3' (qPCR) | |
| HAX1 variant III | 5'-GACCTCGGAGGTGAGA-3' | 5'-CCATATCGCTGAAGATGCTA-3' |
| GenBank: EU190983 | ||
| HAX1 variant IV | 5'-AGGAATTTGGCTTCGGCTTC-3' [26] | 5'-TGCAGAAAGGTGGCAGGTGTT-3' [26] |
| GenBank: EU190982 and HAX1 variant V VEGA:OTTHUMT00000087654 | ||
| CTSD | 5'-GCTGTGAGGCCATTGTGGAC-3' | 5'-GCGACACCTTGAGCGTGTAG-3' |
| GenBank: NM_001909 | ||
| GAPDH | 5'-GGTCGGAGTCAACGGATTTG-3' [27] | 5'-ATGAGCCCCAGCCTTCTCCAT-3' [27] |
| GenBank: NM_002046 | ||
| ACTB | 5'-AGCCTCGCCTTTGCCGA-3' [28] | 5'-GCGCGGCGATATCATCATC-3' [28] |
| GenBank: NM_001101 | ||
Sequences of the primers used in reverse transcription semi-quantitative and quantitative PCR. GeneBank accession numbers are indicated for each transcript except for variant V where transcript ID from Vertebrate Genome Annotation (VEGA) database is shown.