Figure 4.

Multiple climbing fiber innervation of Purkinje cells in mature Gα_q mutant mice. (A) EPSCs elicited by stimulation of climbing fibers (CFs) in the granule cell layer in wild-type (a, b) and Gαq mutant (c, d) Purkinje cells (PCs). Records in a, b, c, and d were taken from mice at postnatal day 50 (P50), P80, P46, and P46, respectively. One or two traces are superimposed at each threshold intensity. Stimuli were applied at 0.2 Hz. Holding potentials were −10 mV for a, b, and c and 0 mV for d. (B) Summary histograms showing number of discrete steps of CF-EPSCs of the wild-type (open columns) and mutant (hatched columns) PCs. Data obtained from mice at P22–P57 (a) and at P72–P109 (b), respectively. Numbers of tested PCs at P22–P57 (a) are: n = 115 (from seven wild-type mice) and n = 124 (from eight Gα_q mutant mice). Those at P60–P109 (b) are: n = 72 (from four wild-type mice) and n = 42 (from three mice). All cells were studied blind to the mouse genotype.