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. 2010 Mar 23;5(3):e9815. doi: 10.1371/journal.pone.0009815

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This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.

This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration, which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.

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293T cells were co-transfected with either HA-wt NS5A or HA-mt NS5A together with FLAG-wt NS5B (Con 1b). The cells were harvested 48 hours after transfection and immunoprecipitated with HA-specific antibody. Immunoprecipitates were western blotted and probed with monoclonal antibody specific for NS5A and anti-FLAG antibody to detect NS5A and NS5B respectively. Signal was detected by chemiluminescence assay (IP-immunoprecipitation, IB-immunoblot).