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. Author manuscript; available in PMC: 2011 Apr 2.
Published in final edited form as: FEBS Lett. 2010 Feb 25;584(7):1386–1392. doi: 10.1016/j.febslet.2010.02.060

Fig 3. Parkin eliminates damaged mitochondria.

Fig 3

(a) 1 hour after mitochondrial depolarization with CCCP, mitochondria (right) are surrounded by Parkin (center) and autophagosomes (LC3, left). (b) The recruitment of autophagosomes to mitochondria is induced by Parkin. In the absence of Parkin expression (left), depolarized mitochondria (red) are not associated with autophagosomes (LC3, green), whereas autophagosomes are more associated with mitochondria in the presence of Parkin (right). Scale: 1 μm. (c) 48 hours after mitochondrial depolarization with CCCP, mitochondria are not detectable with immunostaining. Only cells expressing YFP-Parkin (left), mitochondria (right) are completely eliminated. (d) 48 hours after mitochondrial depolarization with CCCP, mitochondria were taken up by lysosomes only in the HeLa cells expressing YFP-Parkin (right). Scale: 500 nm.