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. Author manuscript; available in PMC: 2011 Apr 1.
Published in final edited form as: Exp Hematol. 2010 Jan 29;38(4):270–281. doi: 10.1016/j.exphem.2010.01.004

Figure 5. Captopril administration to nonirradiated control mice transiently dampens HPC bone marrow progenitor entry into the cell cycle (day 2) followed by a modest increase into G1 to G2 transition (day 7).

Figure 5

Mice were either untreated (control), or treated with captopril (0.1 mg/kg/day) for 2 or 7 days. Femoral bone marrow was obtained and Lin- cells were isolated from pooled femoral bone marrow cells by MACS selection. Cells were stained for Sca-1 and c-Kit cell surface expression. The cell cycle was determined by Ki67-FITC and 7AAD staining using multicolor flow cytometry. Data shown are the mean ± SEM of three separate experiments using pooled marrow from two mice per group, *p < 0.05 from control. (Panel A) Lin− cells (HPC enriched cells). (Panel B) Lin− Sca-1+cKit+ (LSK+, HSC enriched cells).