Table 2.

Molecular masses of Bor1p protein and protein-detergent complex purified in C₁₂E₈ or DDM. Based on the protein sequence, the calculated molecular mass for Bor1p (monomer) including residues of the tag that remain after 3C cleavage is 65.9 kDa.

	Bor1p in DDM	Bor1p in C12E8 (Lipid added after concentration)	Bor1p in C12E8 (Lipid added prior to concentration)
MW Bor1p (TDSEC)a	80 ± 5 kDab	150 ± 20 kDa c	40 ± 20 kDa
MW of complex (TDSEC)d	180 ± 10 kDa	310 ± 30 kDa	320 ± 30 kDa
MW of complex (SEC mobility)e	120 ± 5 kDa	470 ± 30 kDa	690 ± 60 kDa
Detergent/Protein (g/g) (TDSEC)f	1.2 ± 0.2	1.1 ± 0.2	NDg
Detergent/Protein (g/g) (colorimetric)h	1.1	NDi	NDi

^aCalculated using the triple detector method [52]. Values are indicated as mean ± standard deviation.

^bn = 2.

^cn = 25

^dCalculated using the triple detector method [52]using a value of dn/dc for detergent of 0.143 for DDM and 0.109 for C₁₂E₈. In each case, the value of dn/dc for lipid, if present was assumed to be the same as that for detergent.

^eCalculated based on the mobility of the protein containing peak, compared with the mobilities of the standard proteins (see Materials and Methods), n = 3.

^fCalculated as the ratio of the protein and lipid components, each determined by the triple detector TDSEC method.

^gNot determined due to overlap of protein and free lipid/detergent micelle peaks.

^hDetermined by colorimetric assay of fractions from TDSEC chromatography. The amount of detergent in non-peak containing fractions was subtracted from the detergent concentration of the protein-containing peak. The protein concentration was determined based on staining on SDS-PAGE in comparison to known concentrations of purified 3C protease.

ⁱNot determined because the colorimetric assay for detergent is based on detection of sugar moieties and, thus is not applicable to C₁₂E₈.