Figure 1.
Effect of PKA on adenylyl cyclase activities. Adenylyl cyclases were expressed in Hi-5 cells treated without or with PKA and then assayed for indicated activities. (A) Effect of PKA treatment on basal, 20 nM Q227L-Gαs (αs*), and 50 μM forskolin-stimulated AC6 activity. (B) Effect of PKA treatment on basal and 20 nM Q227L-Gαs (αs*) stimulated AC1 and AC2 activities. (C) Effect of varying periods of treatment with PKA on αs*-stimulated AC6 activity. (D) Effect of inclusion of 75 μM WIPTIDE during PKA treatment on basal, 20 nM Q227L-Gαs (αs*), and 50 μM forskolin-stimulated AC6 activity. (E) Effect of PKA treatment on Mn2+-stimulated AC6 activity. Divalent cation concentrations are in excess over 1 mM EDTA and 0.1 mM ATP.