TABLE 1.

Screening Technique	Method of Detecting Ligand Binding	Labeled Protein?	Protein-Ligand Co-structure?	Limited by Protein MW?	Ref.
3-FABS	Chemical shift changes, requires fluorinated ligands	No	No	No	[252]
Affinity NMR	Change in translational diffusion	No	No	No	[174]
AIDA-NMR	Line-broadening change (T2) due to protein-protein complex formation, labeled protein or Trp reporter in ligand binding site	Yes/No	Yes/No	Yes	[253, 254]
FAXS	Line-broadening change (T2) due to ligand competition, requires fluorinated ligands	No	No	No	[255]
MS/NMR	Retention on size-exclusion column & chemical shift changes	Yes	Yes	Yes	[256]
Multi-Step NMR	Line-broadening change (T2) & chemical shift changes	Yes	Yes	Yes	[237]
NOE pumping	Transfer nuclear Overhauser effect (NOE)	No	No	No	[183]
RAMPED-UP NMR	Chemical shift changes, screening multiple proteins	Yes	No	Yes	[257]
SAR by NMR	Chemical shift changes	Yes	Yes	Yes	[258]
SLAPSTIC	Line-broadening change (T2) due to protein spin label	Yes	No	No	[259]
STD NMR	Saturation transfer difference from protein	No	No	No	[181]
WaterLOGSY	Saturation transfer difference from solvent	No	No	No	[182]