Figure 2.

DAT−LeuT conservation of putative vestibular binding pocket residues. (a) The DAT comparative model (lines) and an X-ray structure-based LeuT model (sticks) are superposed with docked clomipramine (cyan/blue, ball-and-stick). Residues within 5 Å of the ligand are pictured; a rmsd correlation matrix of approximately 1 Å was observed. (b) Pairwise sequence alignment and consensus calculation of putative binding pocket residues. For residues in analogous positions, sequence identity (green) and divergence (pink) is indicated in both panels. Analogous residues that are nonidentical but similar with respect to hydrophobic, aromatic, or polar features are indicated (#).