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Initial DAT binding affinity and dopamine uptake inhibition screening for MI-1 to MI-10 at N2A neuroblastoma cells stably expressing WT hDAT. Representative relative DAT binding affinities assessed via [3H]-WIN 35,428 displacement when MI-1 to MI-10 (10 μM) were co-incubated (upper panel) or preincubated for 10 min (middle panel) with the radioligand. Assessment of [3H]-dopamine uptake inhibition by MI-1 to MI-10 (10 μM) (lower panel).
