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. 2010 Feb 23;102(6):1037–1043. doi: 10.1038/sj.bjc.6605573

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Copyright © 2010 Cancer Research UK

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Cyclophilin A (PPIA) C_t variations in PNT2, LNCaP, MCF-7 and HEK cell lines according to hypoxic culture conditions (1, 5, 10 and 20% oxygen). cDNA (500 ng) –provided by reverse transcription of 500 ng of total RNA – was systematically used for qRT-PCR.