Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2010 Mar 2;102(6):1052–1060. doi: 10.1038/sj.bjc.6605591

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2010 Cancer Research UK

This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material.If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit https://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Morphology and motility of F9 and G3 cells. (A) Analysis of the expression of GFAP and vimentin in F9 and G3 cells. Anti-vimentin and GFAP antibodies were used to label F9 and G3 cells. The proteins were visualised (green) by an FITC-conjugated secondary antibody under confocal microscope. Nuclei were visualised in blue with DAPI. (B) Motility in wound-healing model. F9 and G3 cells grown to confluence on plastic were scratched to create a wound. Cells in 10% FCS/DMEM were photographed at 0 and 8 h to assess the mean distance (mm) of leading cells moving towards the ‘wound’ area. ^*Significantly slower locomotion of G3 cells (P<0.01) as compared with F9 cells. (C) Invasiveness of xenograft tumours. F9 and G3 cells at 5 × 10⁶ cells in 100 μl PBS per mouse were injected subcutaneously into the flanks of athymic mice. After 30 days, the xenograft tumours were sectioned and stained by HE. Arrows indicate F9 tumour intruding mouse dermis and well-encircled G3 tumour.