. 2010 Mar 3;65A(4):344–352. doi: 10.1093/gerona/glq018

Table 1.

Sequences of Primers for Real-Time Polymerase Chain Reaction

Gene	Accession Number	Primer Sequences (forward and reverse)
β₂-Microglobulin	NM_009735	F: 5′-AAGTATACTCACGCCACCCA-3′
β₂-Microglobulin	NM_009735	R: 5′-CAGGCGTATGTATCAGTCTC-3′
Insulin receptor	NM_010568	F: 5′-CTTGGACAACCAGAACCTGA-3′
Insulin receptor	NM_010568	R: 5′-CCTTAGTTCCGGAGACTTCT-3′
IGF-1	NM_010512	F: 5′-CTGAGCTGGTGGATGCTCTT-3′
IGF-1	NM_010512	R: 5′-CACTCATCCACAATGCCTGT-3′
IRS-1	NM_010570	F: 5′-AGCCCAAAAGCCCAGGAGAATA-3′
IRS-1	NM_010570	R: 5′-TTCCGAGCCAGTCTCTTCTCTA-3′
PPARγ	NM_011146	F: 5′-GTCAGTACTGTCGGTTTCAG-3′
PPARγ	NM_011146	R: 5′-CAGATCAGCAGACTCTGGGT-3′
PGC1-α	NM_008904	F: 5′-TACGCAGGTCGAACGAAACT-3′
PGC1-α	NM_008904	R: 3′-ACTTGCTCTTGGTGGAAGGA-3′
GLUT2	NM_012879	F: 5′-GGTCAGAACTACCTCTCTTTG-3′
GLUT2	NM_012879	R: 5′-GTGTGTGTGGAATTGTCCTC-3′

Note: GLUT2 = glucose transporter 2; IGF-1 = insulin-like growth factor-1; IRS-1 = insulin receptor substrate-1α; PGC-1α = PPARγ coactivator-1α; PPAR = peroxisome proliferator–activated receptor.