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Immunofluorescence staining of A. thaliana microtubules with antibodies to β-tubulin. Fixed (A, C) or unfixed, detergent-extracted (B, D) preparations of primary root epidermal cells were prepared as described in Material and Methods and stained with monoclonal antibodies 18D6 (A-B) and TU-06 (C-D). Bar, 10 μm.
