Figure 2.

Purification of STIL. A, Chromatograph of the C18 reverse-phase semi-preparative column. The sample was loaded with 6% acetonitrile (MeCN) and eluted with 50% and 100% acetonitrile (indicated by dashed lines). Fractions were assayed for LePRK2 dephosphorylation activity (inset). The position of LePRK2 is indicated by an arrow. B, Purification protocol used for the isolation of STIL from tobacco stigma/style exudates. C, Chromatograph of the second Mono Q column. Abs280 nm (left vertical axis, solid line) and % of Buffer (100% Buffer corresponding to 1 M NH₄HCO₃; right vertical axis, dashed line). Fractions 3 to 27 were assayed for LePRK2 dephosphorylation activity (inset).