Table 1.

Purification table.

Purification Step	K2D50	% Error	Purification Factor
Exudate	16.38	0.4	1.0
Chloroform supernatant	9.78	21.8	1.7
1st MonoQ*	7.35 × 10-04	22.9	22,276
C18*	1.28 × 10-03	18.6	12,769
2nd MonoQ	1.04 × 10-04	+	156,849

(*) No significant difference in a t-test (p > 0.05).

(+) No duplicates available.

K2D₅₀ represents Abs280 units necessary for 50% dephosphorylation of LePRK2; values were obtained from an average of two independent purifications. % Error is calculated as (Standard error/K2D₅₀) × 100. The purification factor was calculated by comparison to the starting exudate.