Table 2.
Biochemical characterization of STIL.
| Treatment | LePRK2 dephosphorylation activity |
|---|---|
| 1.5 N HCl (microwave) | - |
| 1 N HCl 100°C 4 or 20 h | + |
| 1 N NaOH 100°C 2 h | + |
| 50 mM DTT 100°C 15 min | + |
| Pepsin | + |
| Carboxypeptidase Y | + |
| Trypsin | + |
| Proteinase K | + |
| Lyticase | + |
| Pectolyase | + |
STIL was subjected to the listed treatments and the dephosphorylating activity was tested in a LePRK2 dephosphorylation assay (as described in Methods). STIL resistance to the treatment is indicated by "+", while "-" indicates STIL loss of activity.