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Immunodetection of the three DnaJ proteins AtJ8, AtJ11 and AtJ20 in chloroplasts. Chloroplasts were isolated from the leaves of WT and respective mutants after 3 h treatment in darkness. Total chloroplast proteins were used for immunoblotting, and for immunodetection of the AtJ8 protein, 30 μg protein was loaded whereas for immunodetection of AtJ11 and AtJ20 proteins, 100 μg protein was loaded. WT, wild-type.
