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. Author manuscript; available in PMC: 2010 Mar 23.
Published in final edited form as: Ann Neurol. 2009 Apr;65(4):474–479. doi: 10.1002/ana.21605

Table 2.

Quantitative Polymerase Chain Reaction Analysis of Varicella Zoster Virus DNA in Acute Multiple Sclerosis Plaques, in Cerebrospinal Fluid of Patients with Multiple Sclerosis, Clinically Isolated Syndrome, and Other Inflammatory Neurological Diseases, and in Ultracentrifuged Pellets Obtained from Varicella Zoster Virus–Infected Cells in Tissue Culture

Sample VZV 63,a copies/ml VZV 21,b copies/ml VZV 31,c copies/ml
MS 1 0 0 0
MS 2 Traced 0 0
MS 3 0 0 0
MS 4 0 0 0
MS 08-1 (remission) 0 0 0
CIS 1 0 0 0
CIS 2 Traced 0 0
CIS 3 Traced 0 0
MS 95-A3 acute plaque 0 ND ND
MS 03-A1 acute plaque 0 ND ND
OIND 06-1 SSPE 0 0 0
OIND 08-1 VZV vasculopathy 0 0 0
OIND 08-5 VZV radiculomyelitis 8,000 14,200 9,710
OIND 04-5 cryptococcal meningitis Traced 0 0
OIND 05-1 neurosyphilis 0 0 0
OIND 05-2 chronic meningitis Traced 0 0
OIND VZV culture supernatant 6.989 E6 18.7 E6 291,500
a

Open reading frames (ORF) 63 primers sensitive to detect 10 copies varicella zoster virus (VZV) DNA.

b

ORF 21 primers sensitive to detect 400 copies VZV DNA.

c

ORF 31 primers sensitive to detect 40 copies VZV DNA.

d

Trace: amplification at 40 cycles (between 1 and 10 copies) is below the lower limit of reliability for the standard curve with VZV ORF 63 primers.

MS = multiple sclerosis; CIS = clinically isolated syndrome; ND = not done; OIND = other inflammatory neurological disease; SSPE = subacute sclerosing panencephalitis.