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. 2009 Dec 16;29(50):15770–15779. doi: 10.1523/JNEUROSCI.4951-09.2009

Figure 1.

Figure 1.

Simultaneous knockdown of PSD-95 and SAP102 reduces evoked EPSC amplitude in E8 CG neurons. Neurons transfected with the indicated constructs were patch clamped in the whole-cell configuration while stimulating presynaptic neurons with a bipolar extracellular stimulating electrode (arrows) to evoke maximal EPSCs. Dispersion of PDZ scaffolds with CRIPT reduced EPSC amplitude. RNAi knockdown of individual PSD-95 family members had no effect. Simultaneous knockdown of PSD-95 and SAP102, but not other combinations of PSD-95 family members, reduced EPSC amplitude. Values represent the mean ± SEM with n = 8–23 cultures per condition; n = 74 for controls. *p ≤ 0.05 by ANOVA with Dunnett's post hoc test.