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. 2009 Dec 16;29(50):15770–15779. doi: 10.1523/JNEUROSCI.4951-09.2009

Figure 3.

Figure 3.

PSD-95/SAP102 knockdown diminishes spontaneous mEPSC frequency and PPD. A, Left, Whole-cell patch-clamp recordings of mEPSCs in CG neurons in 1 μm TTX. Right, Overlay of 10 consecutive mEPSCs (gray) and the resulting mean (black) from the cells yielding the recordings. B, Knockdown of PSD-95 and SAP102 (P95/S102-RNAi), but not SAP97 (S97-RNAi), decreased mean mEPSC frequency in CG neurons (*p ≤ 0.05 by ANOVA with Dunnett's post hoc test). C, PSD-95/SAP102 knockdown also decreased mEPSC amplitude as seen in cumulative histograms (Cum Fraction; p ≤ 0.01 by K-S test; 9–15 cultures/condition). D, Paired responses in patch-clamped CG neurons exhibited PPD when presynaptic inputs were stimulated (arrows) at an interval of 20 ms. E, Neurons expressing PSD-95/SAP102-RNAi exhibited less PPD than did control neurons, whereas PPD was unchanged in neurons expressing SAP97-RNAi (*p ≤ 0.05 by unpaired Student's t test; 11–15 cultures/condition).