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. 2009 Dec 16;29(50):15770–15779. doi: 10.1523/JNEUROSCI.4951-09.2009

Figure 7.

Figure 7.

SAP102 requires a GK domain to enhance nicotinic synaptic input. A, Left, Evoked EPSCs. Right, SAP102-GFP (S102-GFP) increased the mean evoked EPSC amplitude while SAP102/GK-GFP (S102-GK) reduced it (6–9 cultures/condition). B, Left, Proximal neurites of transfected neurons (green) immunostained for surface α3*-nAChRs (red) and the presynaptic marker synaptotagmin (blue). Arrows indicate examples of receptor/synaptotagmin alignment. Right, SAP102-GFP expression increased the number of α3*-nAChR clusters aligned with presynaptic specializations (Syn α3*-nAChR Clust); SAP102/GK-GFP decreased it (4 cultures/condition). C, Left, Responses to paired stimuli. Right, SAP102-GFP increased PPD (decreased PPR) while SAP102/GK-GFP decreased PPD (increased PPR; 7 cultures/condition). D, Left, Whole-cell responses to nicotine. Right, SAP102-GFP did not alter whole-cell responses to nicotine (5 cultures/condition), but SAP102/GK-GFP decreased it (6 cultures/condition). *p ≤ 0.05; ***p ≤ 0.001 by unpaired Student's t test.