TEWL was measured before and at 0, 3, 24, and 48 hours after acute barrier disruption by repeated Cellophane tape stripping on hairless mouse flanks. Similarly, single application of either LBA (a) or GL (b) 10% immediately after tape stripping to hairless mouse flanks significantly accelerated barrier recovery kinetics 3 hours after disruption in comparison with nLBA, air exposed (AE), V, or nGL. Results shown in as the mean ± SEM (n = 4–6).