Figure 2.

We confirmed the increase in collagen content in the lungs of the mice treated with bleomycin by western blot. Lung lysates (20 μg protein per lane from 6-month-old SAMP8 and SAMR1 mice) were run by using 7% sodium dodecyl sulfate-polyacrylamide gel electrophoresis and then transferred to nitrocellulose membranes, and collagens I and IV was determined by using specific antibodies. There is an important increase in the levels of collagen I in both groups treated with bleomycin but higher in the SAMP8 group. When the content of collagen IV was determined, we detected increase in the SAMP8 group. Note: SAMP = senescence-accelerated prone mice; SAMR = senescence-accelerated resistant mice.