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. 2009 Apr 9;64A(7):731–739. doi: 10.1093/gerona/glp040

Figure 5.

Figure 5.

Expression of SDF-1 increases with age but the ability of BMDMSCs to respond decreases. (A) SAMP8 mice were intratracheally treated with 4 U/kg of bleomycin at the moment of sacrifice 7 and 14 days after injury, and then, samples of peripheral blood were collected. Levels of SDF-1 were measured by enzyme-linked immunosorbent assay and concentrations expressed in ng/mL. There were significant differences in the SDF-1 levels in serum between the 6-month and 12-month samples. (B) To evaluate the ability of BMDMSCs to respond to SDF-1, BMDMSCs were obtained from 12-month-old SAMP8 and SAMR1 mice. Cells were exposed for 2 hours to 200 ng/mL of SDF-1 and the number of cells that migrated was quantified using a hemocytometer. Values represent mean ± standard error. Notes: BMDMSC = bone marrow–derived stem cell; SAMP = senescence-accelerated prone mice; SAMR = senescence-accelerated resistant mice; SDF = stromal cell–derived factor. n = 4. *p < .05 and **p < .001.