Figure 1.

Tissue distribution of lipopolysaccharide (LPS)-induced interleukin-6 (IL-6) gene expression. Systemic inflammation was induced in young (6–7 mos old) and aged (22–27 mos old) C57BL/6 male mice by LPS injection (2.5 mg/kg, intraperitoneal) and mice were sacrificed 6 h later. All lanes are in a single gel. (A) RNA was isolated from various tissues of young and aged mice and analyzed by Northern blotting. Each lane represents pooled RNA samples from three mice. (B) Densitometric analysis of short exposure from (A). The shorter exposure was used because the bands for heart and fat appear to be saturated in the longer exposed autoradiograph. Young (open bars) and aged (closed bars). Total intensity of each band was normalized to 18S levels. The normalized IL-6 messenger RNA level in aged mouse fat was set at 1.0.