Figure 4.

Immunohistochemical localization of interleukin-6 (IL-6) in the adipose tissue. Immunohistochemical analysis was performed to localize IL-6 protein in adipose tissue from aged C57BL/6 male mice after lipopolysaccharide (LPS) injection (2.5 mg/kg, intraperitoneal). Strong positive staining with anti-IL-6 antibody is seen in adipocytes (closed arrow heads), vascular endothelial cells (open arrow heads), and inflammatory cells (arrows) of white epididymal adipose tissue from mice injected with LPS (top middle panel). Strong positive staining is seen in brown perirenal adipose tissue (closed arrow heads) associated with the kidney (Kd, top right panel), but not in vascular endothelial cells of the blood vessels (BV) in mice injected with LPS.