FIGURE 1.

Mitochondrial PTP gating induced by M analogs in Jurkat cells. A, percent TMRM fluorescence intensity of Jurkat cells incubated with increasing M concentrations as indicated. The mean TMRM fluorescence intensity of non-treated Jurkat cells is defined as 100%. Values are mean ± S.E. of three independent experiments. *, significant as compared with non-treated cells (p < 0.05). Inset: representative histogram of cells treated with 0 (a), 100 μm (b), 140 μm (c), or 200 μm of added M (d). B, percent TMRM fluorescence intensity of M-treated Jurkat cells, in the absence or presence of 6.0 μg/ml CsA or 1.0 μm SfA added 1 h prior to TMRM measurement, as indicated. The mean TMRM fluorescence intensity of non-treated Jurkat cells is defined as 100%. Values are mean ± S.E. of three independent experiments. *, significant as compared with non-treated controls (p < 0.05). Inset: representative histogram of non-treated cells (a), M-treated cells (b), and M-treated cells in the presence of added CsA (c).