FIGURE 6.

M-induced apoptosis. A, representative scattergrams of FITC-VAD-FMK and propidium iodide (PI)-bound Jurkat cells. Non-treated controls (a), cells treated for 24 h with 200 μm of M (b), 1 μm SfA (c), or M plus SfA (d). B, relative FITC-VAD-FMK fluorescence intensity of the upper right (late apoptotic cells) plus lower right (early apoptotic cells) quadrants of respective scattergrams. Jurkat cells were treated for 24 h with 200 μm of M, 1 μm SfA, 10 μm Forskolin (Fors), M plus SfA, or M plus Forskolin. The mean FITC-VAD-FMK fluorescence intensity of respective non-treated controls is defined as 1. Values are mean ± S.E. of three independent experiments. *, significant as compared with respective non-treated controls (p < 0.05). C, PARP (89 kDa)/PARP (116 kDa) ratio of COS-1 cells transfected with GFP-Raf1 plasmid or with the respective GFP plasmid and incubated for 24 h with 300 μm of M, as indicated. PARP (89 kDa)/PARP (116 kDa) ratio of extracts of non-treated controls is defined as 1.0. Values are mean ± S.E. of three independent experiments. *, significant as compared with non-treated controls (p < 0.05).