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. 2010 Jan 11;285(10):6952–6959. doi: 10.1074/jbc.M109.055731

FIGURE 1.

FIGURE 1.

EGFR endocytosis and recycling in DGKδ-deficient cells. A, HeLa cells were transfected with Myc-tagged wt or T654A EGFR, and then DGKδ was knocked down using siRNA. After 24 h EGFR (anti-Myc), DGKδ, and actin were detected by immunoblotting. EGFR band densities (arbitrary units) are shown above the blot. B, the band densities of wtEGFR or T654A EGFR from three experiments similar to Fig. 1A were determined and plotted. Shown are mean values with S.D. (*, p < 0.05). B, HeLa cells were transfected with scrambled control or DGKδ siRNA duplexes, and the endocytosis rate constant (ke) was determined using [125I]EGF (11). Shown are mean, relative ke values (±S.D.) from four experiments. The asterisk indicates a statistically significant difference compared with scrambled control cells (p < 0.01). C, HeLa cells were transfected with scrambled control or DGKδ siRNA duplexes, and then recycling of EGFR was measured (12). Shown are mean values (±S.D.) from three experiments.