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. 2009 Dec 18;285(10):6980–6986. doi: 10.1074/jbc.M109.065987

FIGURE 3.

FIGURE 3.

MTA1 is required for HBx-induced iNOS activity. A, quantitative PCR analysis of iNOS and MTA1 mRNAs in HBx-expressing HepG2 cells or control-transfected HepG2 cells with or without MTA1 knockdown by siRNA-MTA1. Control siRNA was used as indicated in the experiments. Expression levels of iNOS were normalized using iNOS amplicon, and MTA1 levels were normalized with β-actin. Results are presented as mean ± S.E., n = 3, with p < 0.001 considered to be statistically significant. B, representative Western blot analysis of iNOS, HBx, and MTA1 in HepG2 cells transfected with increased amounts (100, 250, and 500 ng/reaction) of either control vector (lanes 1–3) or HBx expression vector (lanes 4–6). HepG2 cells with MTA1 knockdown by siRNA-MTA1 (lanes 7 and 8) were transfected with (250 ng/reaction) of either control (lane 8) or HBx expression vector (lane 7). β-Actin was used as an internal control.