Skip to main content
. 2009 Dec 18;285(10):6980–6986. doi: 10.1074/jbc.M109.065987

FIGURE 4.

FIGURE 4.

Selectively knocking down MTA1 compromises iNOS expression. A, HepG2 cells with or without MTA1 knockdown by siRNA-MTA1 were co-transfected with HBx and iNOS promoter luciferase reporter construct. Cell lysates were analyzed for iNOS and MTA1 protein expression. Vinculin was used as an internal control. B, promoter activity was assessed 48 h after transfection. Results are presented as -fold change compared with control (mean ± S.E., n = 3). C, wild type MEFs (■) or MTA1−/− MEFs (□) were co-transfected with HBx and iNOS luciferase reporter construct. iNOS promoter activity was assessed after 48 h. Results are presented as -fold change compared with control (mean ± S.E., n = 3). D, cell lysates were analyzed for iNOS protein expression by Western blot. β-Actin was used as a loading control.