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. 2009 Dec 18;285(10):6980–6986. doi: 10.1074/jbc.M109.065987

FIGURE 5.

FIGURE 5.

MTA1-HBx protein complex interacts with NF-κB Sequence of the iNOS gene promoter. A, recruitment of HBx or MTA1 to iNOS chromatin (−1 to −246, −5344 to −5593, and −5707 to −5941) by ChIP assay in HepG2 cells after being transfected with either pCMV vector control or pCMV-HBx. B, recruitment of HBx followed by MTA1 to iNOS chromatin after HBx-expressing cells were treated with parthenolide (5 μm). Recruitment of HBx followed by MTA1 to iNOS chromatin (−1 to −246 and −5344 to −5593) was analyzed by sequential double ChIP assay in HepG2 cells. C, nucleus extracts of HBx and control vector transient transfected cells (2000 ng/reaction) were subjected to EMSA. A PCR product of the iNOS promoter region encompassing the functional NF-κB consensus sequence. Probe control (0.3 ng/lane), MTA1 antibody (MTA1 Ab.), and IgG control (1000 ng/lane) were used. Reactions were also carried out in the presence or absence of parthernolide. IP, immunoprecipitation.