FIGURE 6.

Ectopically expressed MTA1 rescued inhibitory effects of parthernolide. A, HBx-expressing HepG2 cells were treated with parthernolide and subsequently transfected with pcDNA-MTA1 control expression vector (500 ng/reaction in a 6-well plate). Cells were then subjected to an iNOS promoter assay. B, subsequently, cell lysates were subjected to Western blot analysis for iNOS expression. Western blot analysis for HBx and T7 tag was carried out for transfection efficiency. Vinculin was used as a loading control. C, the levels of nitrite accumulation/10⁵ cells are presented as mean ± S.E., n = 3.