FIGURE 1.
Expression of mBD4 and its human orthologue hBD2 as IgG1-Fc fusion proteins. a, alignment is shown of the predicted amino acid sequence of mBD4 with other human β-defensins using the ClustalW algorithm. Conserved residues among mBD4 and hBD2 are shown in gray, and conserved cysteine residues are highlighted in black. b, schematic drawing of the β-defensin fusion proteins is shown. c, shown is a Western blot analysis of the recombinant expressed mBD4:Ig, hBD2:Ig, and mCCL20:Ig fusion proteins using a peroxidase-conjugated donkey anti-human IgG monoclonal antibody for detection. MW, molecular mass marker.