FIGURE 4.
Competition assays for binding of mBD4:Ig and hBD2:Ig to mCCR6- and hCCR6-expressing cells. HEK293 cells stably transfected with mCCR6 were incubated with 1 μg/ml mBD4:Ig (black line) or 1 μg/ml hIgG1 (filled gray) (a), and cells transfected with hCCR6 were incubated with 1 μg/ml hBD2:Ig (black line) or 1 μg/ml hIgG1 (filled gray) (b). For competition analysis transfected HEK293 cells were pretreated with mCCL20 (a) or hCCL20 (b) 20 min before the addition of β-defensin fusion proteins (dashed line). Binding was determined by flow cytometry using a phycoerythrin (PE)-conjugated anti-human IgG-Fc antibody. Data show one representative experiment of three independent experiments.