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. 2010 Jan 12;285(10):7028–7034. doi: 10.1074/jbc.M109.091090

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© 2010 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 5. — Chemotactic activity of mBD4:Ig and hBD2:Ig for mouse CCR6-expressing cells. The migration of mock transfected HEK293 cells and HEK293 cells stably expressing mCCR6 in response to various concentrations of mCCL20 (a) and recombinant mBD4:Ig, hBD2:Ig and hIgG (b) was tested using a 48-well chemotaxis chamber assay. The migration of HEK293 cells stably expressing mCCR6 in response to various concentrations of recombinant mBD4:Ig (c) and hBD2:Ig (d) after preincubation with mCCL20 (100 ng/ml) or in response to various concentrations of mCCL20 after preincubation with mBD4:Ig or hBD2:Ig (e) was tested using a 48-well chemotaxis chamber assay as described under “Experimental Procedures.” Data are the means ± S.D. of one representative experiment of three, each done in triplicate. HPF, high power field.