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. 2010 Jan 12;285(10):7028–7034. doi: 10.1074/jbc.M109.091090

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© 2010 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 6. — Chemotactic activity of mBD4:Ig and hBD2:Ig for human CCR6-expressing cells. The migration of mock-transfected HEK293 cells and HEK293 cells stably expressing human CCR6 in response to various concentrations of hCCL20 (a) and recombinant mBD4:Ig, hBD2:Ig, and hIgG (b) was tested using a 48-well chemotaxis chamber assay. The migration of HEK293 cells stably expressing human CCR6 in response to various concentrations of recombinant hBD2:Ig after pre-incubation with human CCL20 (100 ng/ml) (c) or in response to various concentrations of human CCL20 after pre-incubation with hBD2:Ig (d) was tested using a 48-well chemotaxis chamber assay as described under “Experimental Procedures.” Data are the means ± S.D. of one representative experiment of three, each done in triplicate. HPF, high power field.