FIGURE 3.

Effect of divalent cations, Mg²⁺ and Mn²⁺, on Her4 kinase domain activation. A, Her4 WT kinase domain was incubated in the presence or absence of the indicated liposomes and the kinase reaction conducted in the presence of the indicated concentration of Mg²⁺ or Mn²⁺. Fold change is calculated relative to the activity in the absence of liposomes. B, Her4 V954R kinase domain was incubated in the absence or presence of nickel-liposomes and the kinase reaction conducted in the presence of the indicated concentration of Mg²⁺ or Mn²⁺. Data are representative of three (A) or two (B) independent experiments.