FIGURE 3.

Absence of CRT expression reduces type I collagen secretion and ECM incorporation. Wild type and CRT^−/− MEFs were cultured for 48 h in 10% FBS and then 72 h in 0.5% FBS. Deoxycholate was used to separate the cell fraction (DOC-soluble) from the ECM fraction (DOC-insoluble). The entire ECM fraction and 1/10th of the cell fraction were separated by SDS-PAGE. A, after transfer, membrane was immunoblotted (IB) for type I collagen. Blots were stripped and reprobed with an antibody to total ERK. A representative blot is shown (n = 3 separate experiments). B, gel was stained with Coomassie Blue to detect total protein in each sample.