FIGURE 9.

Absence of CRT does not alter collagen chaperone HSP47 or prolyl 4-hydroxylase expression. Wild type and CRT^−/− MEFs were grown to confluence. Cells were harvested in reducing Laemmli sample buffer. Equal volumes of lysates were separated by 10% SDS-PAGE, transferred to nitrocellulose, and immunoblotted with rabbit anti-CRT polyclonal antibody, mouse anti-HSP47 monoclonal antibody, and goat anti-prolyl 4-hydroxylase antibody. Membranes were stripped and reprobed with rabbit anti-β-tubulin.