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APOBEC2 expression increases during myoblast differentiation in vitro. Primary mouse myoblasts were cultured under low serum conditions to induce muscle cell differentiation. At days 1–5 after the start of serum starvation, the ratio of multinucleated myotubes to mononucleated myoblasts (fusion index) was determined, and cell extracts were analyzed by Western blotting for expression of APOBEC2 (A2), myosin HC (MyHC), and histone H3.
